ABSTRACT
Five monoterpenoid compounds(1-5) were isolated and purified from the acetone fraction of the aqueous extract of Zingiberis Rhizoma Recens by MCI, Sephadex LH-20, silica gel, semi-preparative HPLC, and TLC. Their structures were identified with multiple spectroscopical methods including 1 D-NMR, 2 D-NMR, and MS. The five compounds were identified as(2E,6Z)-8-hydroxy-2,6-dimethylocta-2,6-dien-1-yl-(E)-3-(4-hydroxy-3-methoxyphenyl) acrylate(1),(2E,6E)-8-hydroxy-3,7-dimethylocta-2,6-die-noic acid(2),(E)-1,8-dihydroxy-3,7-dimethyl-2-octenoic acid(3), linalyl-β-D-glucopyranoside(4), and β-D-glucopyranoside-(2E)-3,7-dimethyl-2,6-octadien-1-yl(5), respectively.Compound 1 was a new monoterpene ester, and compounds 4-5 were isolated from this plant for the first time.
Subject(s)
Chromatography, High Pressure Liquid , Esters , Monoterpenes , RhizomeABSTRACT
Objective·To investigate the clinical significance and predictive value of human papillomavirus (HPV) 16/18 E6 protein in the different cervical intraepithelial neoplasia. Methods·The expression of HPV16/18 E6 in 10 normal cervical tissues, 33 cervical intraepithelial neoplasiaⅠ (CINⅠ ), 31 CINⅡ- Ⅲ, 30 cervical cancers was detected by immunohistochemistry, explored the expression difference and the relationship with the clinicopathological characteristics of cervical cancer and the prognosis of different CIN. Results·The positive expression rates of HPV16/18 E6 in normal cervical tissues, CINⅠ , CINⅡ - Ⅲ and cervical cancer group were up-regulated (χ2=19.82, P=0.000). HPV16/18 E6 increased positive expression rates in the low grade and the big size tumors of cervical cancer tissues were detected (P=0.033, P=0.011). There were positive correlations between the overexpression and the pathological grade, tumor size, poor prognosis of cervical cancers respectively (r=0.456, P=0.011; r=0.578, P=0.000; r=0.645, P=0.000).The sensitivity,specificity,and diagnostic accuracy rates of HPV16/18 E6 positive expression to the progression of CINⅠ,CINⅡ-Ⅲand cervical cancer were respectively 100.00%, 62.50%, 43.75%; 96.77%, 91.30%, 92.86%; 96.97%, 83.87%, 66.67%. Conclusion·HPV16/18 E6 overexpression plays an important role in the generation, development and the poor prognosis of cervical cancer. HPV16/18 E6 has a good predictive value for the prognosis and hierarchical management of cervical diseases.
ABSTRACT
Objective: To investigate the effect of peiminine (PMI) on reversing the multi-drug resistance (MDR) of gastric cancer cell and enhancing the antitumor activity of adriamycin (ADR) on SGC7901/VCR cell xenograft and to explore its functional mechanisms. Methods: The model of SGC7901/VCR cell line xenograft on athymic mice was established, and the mice were randomized to groups with ip injection of different drugs once every other day, for six times in all. The tumor volume and the body weight of mice were measured during the drug therapy. The mice were sacrificed after the last administration. The tumors were weighed and then the inhibitory rate was calculated. Western blotting was employed to detect the expression of P-glycoprotein (P-gp) and Cleaved Caspase-3. Results: During the treatment, the tumor volume of mice in PMI + ADR group was decreased significantly compared with the control group (P < 0.01). At the end of the treatment the mice were sacrificed and the tumor weight was measured. The tumor weight and inhibitory rate of mice in PMI + ADR group were obviously decreased compared with the control group (P < 0.05). The expression of P-gp in tumor of mice in ADR + PMI group was decreased. On the contrary, the expression of Cleaved Caspase-3 was increased. Conclusion: The PMI is able to enhance the sensitivity of drug resistance on gastric cancer cell to adriamycin and its mechanism may be related to decreasing the expression of P-gp and increasing the expression of Cleaved Caspase-3 in tumor tissues.